Fig. 2

Transcriptome analysis revealed the upregulation of MMP8 in both CEBPD expression and ouabain treatment in astrocytes. A Overexpression of CEBPD and B treatment with ouabain in human primary astrocytes exhibited changes in the gene expression profile, with profile genes selected based on a significance threshold of padj > 0.05. C Venn diagrams and D a heatmap illustrated the correlation of the five upregulated genes. The number on the heatmap represents the Log2 expression value. All Next-Generation Sequencing (NGS) analyses were conducted using cells collected from a single experiment to unveil these findings. Subsequently, the analyzed data was validated through a Q-PCR assay to ensure accuracy