Fig. 3
BBG treatment attenuated P0180 − 199 induced EAN. P0180 − 199 in complete Freund’s adjuvant was injected into Lewis rats (day 0) to immunize them. (A) Neurological function scores of rats in the prevention, treatment, and vehicle groups from day 7 to day 28 post-injection. (B-C) BBG treatment delays onset in the prevention group of EAN rats and lowers the maximum scores in EAN rats. (D) Hematoxylin and eosin staining and LFB staining of EAN rat sciatic nerve were performed to assess inflammatory cell infiltration and demyelination, respectively Demyelination is indicated by arrows; the demyelinated lesion is lighter or absent of blue staining. Representative photomicrographs are shown for each group. Scale bars, 10 μm. (E) Mean number of inflammatory cells per square millimeter of tissue section. (F) Degree of demyelination, as indicated by the histological scores. Experiments were performed in triplicate (n = 6 per replicate) with similar results. BBG-P, preventative BBG group; BBG-T, therapeutic BBG group. *p < 0.05, **p < 0.01