Fig. 1
From: Changes in lipid metabolism track with the progression of neurofibrillary pathology in tauopathies
Neurofibrillary pathology progressively increased in an age-dependent manner in the brainstem of Tg rats. A-B, Immunohistochemical staining (DAPI-blue, pSer202/pThr205-green) demonstrated the presence of tau protein phosphorylated at pSer202/pThr205 in the medulla oblongata and pons of Tg rats. C-F, Progressive increase in the levels of sarkosyl-insoluble tau protein complexes in the medulla oblongata and pons of Tg rats. G-H, Increase in soluble hyperphosphorylated truncated tau in medulla and pons of Tg rats. Ontogenesis of soluble tau and sarkosyl-insoluble tau complexes in aging rats from 4 to 14 months (n = 6 for each age) was monitored by Western blot analysis using phosphorylation-dependent anti-tau antibodies against pThr212, pThr181, pThr217, pSer199, pThr231, pSer262 and pSer202/pThr205. Total tau was determined with a DC25 anti-tau antibody. I-J, The levels of biofluid markers (neurofilament and total tau) of tau pathology. K, The summary of changes in tau pathology during the lifespan of the Tg rats. Data are presented as boxplots (Min to Max)