Fig. 2

Overview of experimental approach, targeted lipidomic and metabolomic analyses, and data processing. A Experimental workflow started with the collection of brain tissue, CSF, and plasma samples of 4, 6, 8, 10, 12, and 14 months-old transgenic (SHR-24) and age-matched controls (SHR). Subsequently, high-coverage lipidomic and metabolomic analyses were performed together with biochemical analysis for tau pathology characterization. Two omics datasets were processed and statistically evaluated together using several approaches. B Overview of several identified lipids and metabolites in brain tissue, CSF, and plasma. Lipids were grouped according to the LIPID maps classification system (fatty acids, glycerolipids, glycerophospholipids, sphingolipids, and sterols). Metabolites were grouped according to their chemical structure into 7 main groups (acylcarnitines, amines, amino acids, and derivatives, coenzymes, and vitamins, organic acids, purine & pyrimidine metabolites, saccharides, and derivatives). C Venn diagram shows the numbers of identified lipids and metabolites in each compartment