Fig. 4

Global effects of brain aberrant metabolism on the composition of CSF. Univariate and multivariate analyses of metabolic patterns found in cerebrospinal fluid of 10-month-old SHR-24 Tg rats compared with age-matched controls. A Principal component analysis (PCA), and orthogonal partial least square discriminant analysis (OPLS-DA). B Metabolic map showing results from significance testing for lipids – student’s t-test (p-value and fold change). C Age-related changes of the most distinctive lipid classes (data are presented as median intensities with 95% confidence intervals). D Lipid ontology enrichment analysis. E Metabolic map showing results from significance testing for metabolites – student’s t-test and fold change. F Enrichment pathway analysis of metabolomics data. The biochemical pathways were evaluated using p-value and enrichment ratio. G Age-related changes of metabolites belong to the pathways: purine catabolism (xanthine, uric acid) and analytes of creatine/arginine metabolism (creatine, phosphocreatine, arginine), free carnitine, myoinositol and citrate + isocitrate (data are presented as median intensities with 95% confidence intervals). Abbreviations LPE – lysophosphatidylethanolamines, PE – phosphatidylethanolamines, PS – phosphatidylethanolserines, PC – phosphatidylcholines, LPC – lysophosphatidylcholines, SM – sphingomyelins, CER – ceramides, HCER – hexosyl-ceramides, H2CER – dihexosyl-ceramides, DAG – diacylglycerols, TAG – triacylglycerols, PI – phosphatidylinositols, PG – phosphatidylglycerols, CE – cholesteryl esters, FA – fatty acids, PCO – plasmenyl phosphatidylcholines, LPCO – plasmenyl lysophosphatidylcholines, PEO – plasmenyl phosphatidylethanolamines, LPEO – plasmenyl lysophosphatidylethanolamines