Fig. 7
From: Changes in lipid metabolism track with the progression of neurofibrillary pathology in tauopathies
Lipid droplet accumulation in microglia of Tg rats and human tauopathies. A Representative confocal images. LDs accumulate in microglia of SHR-24 Tg rats. BODIPY+(green) and IBA1+(red) in the brainstem (medulla oblongata, pons) of 12-month-old SHR-24 Tg rats. Scale bar 20 μm. B Quantification of BODIPY+ cells (CN: 1.2 ± 1.9; TG: 3.5 ± 2.5; p = 0.0214; n = 10, mean ± SD; student’s t-test with p-value). C-D LDM isolated from brain tissue of control and Tg animals. Representative images of BODIPY+ (green), and Mitotracker (red) staining in LDM. E Luciferase luminescence ATP detection assay showed decreased ATP levels in the LDM sample of Tg rats compared to controls. Data are presented as boxplots (Min to Max), student’s t-test with p-value. F LD formation in human brain tissue of patients with PSP and CBD. Oil Red O staining neutral lipids images. G Quantification (CN: 1.46 ± 0.53; PSP: 18.30 ± 7.27; p < 0.0001 CBD: 26.30 ± 6.96; p < 0.0001; n = 5, student’s t-test with p-value). H Representative images of DAPI (blue), BODIPY+ (green), and IBA+ (red) staining in the human brain. Scale bar 10 μm. Abbreviations: PSP - Progressive supranuclear palsy, CBD - Corticobasal degeneration