Fig. 2From: The mouse retinal pigment epithelium mounts an innate immune defense response following retinal detachmentTemporal gene and protein expression changes after retinal detachment and sequencing validation. (A) Heatmap showing significantly upregulated (FC ≥ 1.5, padj≤0.05, red) and downregulated (FC ≤ 0.67, padj≤0.05, blue) DEGs 1 and 7 dprd. (B-F) Relative expression values of DEG grouped by temporal expression. (B) Log2FC of all DEGs significantly upregulated at both 1 and 7 dprd (Up/Up). (C) Log2FC of DEGs significantly upregulated only at 1 dprd (Up/NSC). Gray indicates DEG that were not significantly changed (NSC). (D) Log2FC of DEGs significantly upregulated only at 7 dprd (NSC/Up). (E) Log2FC of DEGs significantly downregulated only at 1 dprd (Down/NSC). (F) Log2FC of DEGs significantly downregulated at both 1 and 7 dprd (Down/Down). (G-K) QRT-PCR of temporal DEG expression changes after retinal detachment for RNA-Seq validation. Relative mRNA expression of (G) Grem1, (H) S100a8, (I) Serpine3, (J) Rpe65, and (K) Tsc22d3, obtained from isolated naïve RPE (n = 6), as well RD RPE at 1 dprd (n = 6) and 7 dprd (n = 6). Bar graphs represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with repeated measures followed by Tukey’s post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001Back to article page