Fig. 1
From: CSF1R antagonism results in increased supraspinal infiltration in EAE
CSF1R antagonism depletes microglia at steady state. A Experimental schema depicting that healthy C57BL/6 mice were maintained on control diet (CD) or PLX5622 diet (PD) for seven days. Tissues were then collected and analyzed by flow cytometry and immunohistochemistry. B Flow cytometric analysis shows that both total CNS cells and CD11b+CD45lo (microglia) numbers are reduced in PD compared to CD mice in steady state. C Immunohistochemical staining of cerebellum (left) and spinal cord (right) tissues confirms robust IBA1+ cell depletion in PD mice. Scale bars represent 100 μm. Results are shown as means ± SD, n = 6–7; **p < 0.005, ****p < 0.00005