Fig. 11

CNS infiltration is prominent in the cerebellum of PD mice with EAE. Lumbar spinal cords and cerebella were isolated at the onset of neurological deficits, and analyzed via immunohistochemistry for mean fluorescence intensity (MFI) of CD11b (myeloid cells), and for counts of CD3+ T cells and Ly6G+ neutrophils. A/B Spinal cords of CD mice show increased infiltration by myeloid cells, T cells (A), and neutrophils (B), while these cells are sparsely detected in the parenchyma or in the meninges of the PD spinal cords. Quantification is shown in graphs on the right. C/D Cerebella of CD mice are only mildly infiltrated by myeloid cells, T cells (C), and neutrophils (D), while these cells are found in large numbers in the cerebellum of PD mice. Quantification is shown in graphs on the right. Scale bars denote 100 μm. Both male and female mice were employed. Data are shown as means ± SD, n = 8; at least 2 sections per tissue; *p < 0.05, **p < 0.005, ***p < 0.0005