Fig. 5
From: CSF1R antagonism results in increased supraspinal infiltration in EAE
Impaired T effector cell generation in the SLOs of PD mice in preclinical EAE. A Gating strategy for flow cytometry analysis of T cell subsets in the EAE SLOs B Frequencies and numbers of CD4+ and CD8+ T cells in the SLOs of CD and PD mice. C Frequencies and numbers of MOG-specific CD4 T cell (CD45+CD11b-CD4+) subsets Th1 (IFNγ+), Th17 (IL17+), Th1/17 (IFNγ+IL17+) were decreased in the PD compared to CD SLOs. D Log10 ratios of Th17:Th1 were increased in the SLOs of PD compared to CD. E Both frequencies and absolute numbers of Tregs (CD25+FoxP3+) were decreased in PD SLOs compared to CD. F Ratios of Treg:Teff were increased in PD SLOs compared to CD. Representative flow plots are shown. Both male and female mice were employed. Data are shown as means ± SD, n = 22; **p < 0.005, ***p < 0.0005, ****p < 0.00005.