Fig. 7

CSF1R antagonism increases CNS infiltration by lymphocytes before neurological symptoms appear in EAE. Single cells isolated from pooled brain and spinal cord tissues were analyzed by flow cytometry for T cell subsets before onset of neurological deficits (preonset). A Gating strategy for flow cytometric analysis of T cells in the EAE CNS. B Frequencies of both CD4+, but not of CD8+ T cells decreased, but the numbers of both subsets increased in the CNS of PD compared to CD mice. C Frequencies of Th1 cells were not different between the groups, but their absolute numbers were increased in the CNS of PD compared with CD. Although the frequencies of Th1/17 and Th17 cells were decreased their absolute numbers were not different in the CNS of PD compared to CD mice. D Log10 Th17:Th1 ratios were below 1 in the CNS of PD mice, while in CD they were greater than 1. E Both frequencies and numbers of Tregs were increased in the CNS of PD compared to CD mice. F Treg:Teff ratios were increased in the CNS of PD mice compared to CD before EAE onset. Representative flow plots are shown. Both male and female mice were employed. Data are shown as means ± SD, n = 22; *p < 0.05, **p < 0.005, ***p < 0.0005