Fig. 8

Only mild differences in the myeloid cell populations persist in the CNS of PD mice in acute EAE. Single cells isolated from pooled brain and spinal cord tissues were analyzed by flow cytometry during acute EAE. A Numbers of total cells were lower in the PD CNS compared to CD. There were no differences in the numbers of total infiltrating cells (CD45hi). Although the frequencies of myeloid cells (CD45hiCD11b+) and lymphocytes (CD45hiCD11b-) remained higher in PD compared to CD mice, their numbers were not different between the groups in acute EAE. Microglia frequencies and numbers remained low in PD compared to CD mice. B No differences in frequencies and absolute numbers of neutrophils were detected between the groups at this timepoint. C Although the frequencies of total macrophages (CD45hiCD11b+Ly6G-CD11c-MHCII+) were increased in the PD mice, there were no differences in absolute numbers. No differences were detected in total monocytes (CD45hiCD11b+Ly6G-CD11c-MHCII-) and total DCs (CD45hiCD11b+Ly6G-CD11c+MHCII+) between the groups. D Frequencies of cDCs and moDCs decreased, but their absolute numbers were not different in PD compared to CD. E Frequencies of Ly6C+ monocytes and macrophages were increased while Ly6C- monocytes and macrophages were decreased in the CNS of PD compared to CD mice during acute EAE. Representative flow plots are shown. Both male and female mice were employed. Data are shown as means ± SD, n = 12; *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005