Fig. 9

Mild differences in T cell populations persist in the CNS of PD mice during acute EAE. Single cells isolated from pooled brain and spinal cord tissues were analyzed by flow cytometry during acute EAE. A Although there were no differences in the CD4+ and CD8 + T cell frequencies, the numbers of CD4+ T cells were increased in the CNS of PD mice compared to CD. B Only numbers of Th1 cells remained elevated during acute EAE within the CNS of PD compared to CD mice. C Log10 Th17:Th1 ratios were below 1 for both groups and were significantly lower in the CNS of PD compared to CD mice. D There were no differences in the Treg populations between the groups. E Treg:Teff ratios were decreased in the CNS of PD compared to CD group. Representative flow plots are shown. Both male and female mice were employed. Data are shown as means ± SD, n = 12; *p < 0.05, **p < 0.005, ***p < 0.0005