Fig. 6

ETs orchestrate the inflammatory response to retinal injury. a Tracking of neutrophils (Ly6G⁺) in the same eye of a PAD4 inhibitor-treated mouse during injury response. Persistent number of CD4⁺ and CD8⁺ cells clustered in the damaged site. b Tracking of CD4⁺ and CD8⁺ T-cells in the same eye of a C57BL/6 mouse within the damaged area (magenta circles). The density of CD4⁺ T-cells remained elevated for 4 days, when CD8⁺ cells also clustered in the damaged area. c, d Quantification of the number of neutrophils, CD4 and CD8 T-cells per lesion after laser in the proximity of the NFL (superficial/intermediate VP) and more in-depth by the PR at baseline and at different time points (Da 1, 4, 7, 10 and 14). Significant differences (***p < 0.001 and ****p < 0.0001) between baseline and the different time points were determined by using a post-hoc Bonferroni one-way ANOVA test (n = 8). Field of view is approximately ≈ 575 µm. e Images of the anatomy and flow patterns of the superficial microvasculature obtained from the angiogram of PAD4 inhibitor-treated mice after injury (Day 0) and at different time points (Da 1, 4, 7, 10 and 14). We showed non-flowing capillaries with plugs (arrows) seen as filling defects, compared to day 1 when capillaries were flowing. f Percentage of non-flowing superficial capillaries per lesion after laser analyzed at different time points (Da 1, 4, 7, 10 and 14). Significant differences (*p < 0.1, ***p < 0.001 and ****p < 0.0001) between baseline and the different time points were determined by using a post-hoc Bonferroni one-way ANOVA test (n = 8). g Spearman correlation between capillary plugs per lesion with the number of neutrophils (Ly6G⁺), helper (CD4⁺), and cytotoxic T-cells (CD8⁺) clustering in the damaged site. Combined data from Days 4 and 7 after laser-injury