Fig. 2

Nhe1 cKO specifically expanded a microglial subcluster with increased lipid metabolism, phagocytosis and lysosomal functions in post-stroke white matter tissues. a UMAP of microglial subtypes from CL and IL hemispheres of WT and cKO white matter tissues. b Violin plots of signature genes for homeostatic microglia and disease-associated microglia (DAM) in each microglial subtype. c Trajectory analysis of microglial cells annotated by cell state, microglial subtype, or developmental pseudotime. d Split view of UMAP of microglial cells in CL or IL hemispheres of WT and cKO white matter tissues. e Proportion of microglial subtypes in CL or IL hemispheres of WT and cKO white matter tissues