Fig. 1.

Adiponectin deficiency exacerbates NLRP3-inflammasome activation in 5xFAD mice. A Representative images of co-immunofluorescent staining of NLRP3 and Iba1 in the hippocampus of 3, 9, and 15-month-old 5xFAD and APN^−/−5xFAD mice. (n = 3 mice for each experimental group) (Magnification: 10 × 20; Magnified: 10 × 40; Scale bar: 50μm). B Relative intensity of NLRP3 immunofluorescent staining in the hippocampus. C Western blotting assay of ASC, Pro-caspase-1, and cleaved caspase-1 in the hippocampus of 9-month-old 5xFAD and APN^−/−5xFAD mice. (n = 3 mice for each experimental group). D Densitometric analysis of ASC, Pro-caspase-1, and cleaved caspase-1 levels. E ELISA assay of hippocampal IL-1β of 3, 9, and 15-month-old 5xFAD and APN^−/−5xFAD mice. (n = 3 mice for each experimental group). Data were presented as the mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001. Statistical analysis was performed by unpaired t tests.