Fig. 2

Trimeric adiponectin suppresses Aβ oligomer-induced IL-1β secretion from microglia via NLRP3-inflammsome pathway. A Representative western blotting image indicates pre-treatment of mammalian recombinant trimeric APN protein reduced NLRP3 level in Aβ oligomer-induced microglia. (n = 3 cultures for each group with duplicated experiment). B Densitometric analysis of NLRP3 and α-tubulin levels. C ELISA assay of IL-1β in the microglia BV2 cells. (n = 6 for each group). D ELISA assay of IL-18 in the microglia BV2 cells. (n = 6 for each group). Data were presented as the mean ± s.e.m for at least three independent experiments, and each performed in duplicates (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001. Statistical analysis was performed by one-way ANOVA followed with Bonferroni’s post hoc comparison tests.