Fig. 4

Single dose of AAV2/8-APN^Tri increases trimeric adiponectin in circulation and cerebral adiponectin levels in 5xFAD mice 4 months after injection. A Preclinical study scheme of single dose AAV injection at 4-month-old 5xFAD. B ELISA analysis of plasma APN in wildtype, AAV2/8-GFP-treated, and AAV2/8-APN^Tri-treated 5xFAD 4 month after injection. (n = 6 mice for each experimental group). C Non-denaturing SDS PAGE analysis of plasma APN by anti-flag and anti-APN antibodies indicated the levels of trimeric APN in the circulation 4 months after injecting AAV2/8-GFP and AAV2/8-APN^Tri. (n = 4 mice for each experimental group). D Densitometric analysis of APN^Tri in AAV2/8-GFP-treated and AAV2/8-APN^Tri-treated 5xFAD. E Immunofluorescent staining of APN using anti-flag and anti-APN antibodies in the cerebral cortex of AAV2/8-GFP-treated, and AAV2/8-APN^Tri-treated 5xFAD 4 month after injection. (n = 4 mice for each experimental group) (Magnification: 10 × 20; Scale bar: 50μm). Data were presented as the mean ± s.e.m.; *p < 0.05; **p < 0.01; ***p < 0.001. Statistical analysis was performed by one-way ANOVA followed with Bonferroni’s post hoc comparison tests and unpaired t test.