Fig. 7
Recombinant APN increases microglial phagocytic activities induced by Aβ peptides and oligomers in vitro. A Representative images of FAM-Aβ1-42 phagocytosis in BV2 microglia cells. BV2 cells were pre-incubated with APN for 2 h followed by incubation with FAM-Aβ1-42 (green) for 24 h before staining Iba1 (red). Scale bar = 100 μm. B The bar graphs showed percentage of phagocytic cells Data were presented as the mean ± SEM for at least three independent experiments (n = 3). C Representative images of latex bead phagocytosis in BV2 microglia cells. BV2 cells were pre-incubated with APN for 2 h followed by incubation with AβO for 24 h and then were loaded with fluorescent beads (green) for 1 h at 37 °C before staining Iba1 (red). Scale bar = 100 μm. D The bar graphs show percentage of phagocytic cells Data were presented as the mean ± SEM for at least three independent experiments (n = 3). One-way ANOVA with Tukey’s multiple comparison test revealed difference between groups. *p < 0.05