Fig. 2

The effects of FH535 on subretinal fibrosis, EMT and CNV in laser-induced CNV mice. A, C Immunofluorescence of the whole RBCC flat-mounts showing intravitreal injection of FH535 (0.5 μmol/L) inhibited the degree of subretinal fibrosis and CNV in mice at day 7 post laser induction. The fibrosis and neovascularization were marked by α-SMA, fibronectin, collagen I and IB4. B, D Quantitative analysis of the effects of FH535 on the subretinal fibrotic area and neovascularization area in laser-induced CNV mice. E Immunofluorescence staining of the whole RBCC flat-mounts showing the effects of FH535 on the co-staining of fibrotic indicator fibronectin and RPE marker RPE65 in CNV model mice. F Quantitative analysis of the effects of FH535 on the subretinal fibrotic area and RPE65-positive immunofluorescence area in CNV model mice. Scale bars, 100 µm. α-SMA, alpha-smooth muscle actin; CNV, choroidal neovascularization. Data are expressed as mean ± SEM. ***p < 0.001, ****p < 0.0001 compared with the CNV group