Fig. 3

The impact of intravitreal administration of FH535 or Box5 on Wnt-signaling, EMT and subretinal fibrosis in laser-induced CNV mice. A–D The changes of Wnt-related molecules (Wnt5a, ROR1, and Wnt3a) examined with Western blot in RBCCs from day 3 to day 14 after laser induction in mice with or without FH535 treatment (0.5 μmol/L). E and F The effects of intravitreal injections of FH535 or Box5 (90 μmol/L) on β-catenin and α-SMA expression within the CNV lesions in mice at day 21 after laser induction, along with the corresponding quantitative analysis. G and H The immunofluorescence of fibronectin and RPE65 in RBCC flat-mounts of mice among above different groups. Scale bars, 100 µm. α-SMA, alpha-smooth muscle actin; CNV, choroidal neovascularization; Nor, Normal; ROR1, receptor tyrosine kinase-like orphan receptor 1; RBCC, RPE-Bruch’s membrane-choriocapillaris complex. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with the Nor or CNV group; ^#p < 0.05 compared with CNV group (B and C)