Fig. 5

The impact of FH535 co-incubation on the EMT and migratory capacity of ARPE-19 cells treated with TGFβ1. A Representative bright field images depicting cellular morphology, as well as immunostainings for fibronectin, collagen I, vimentin and ZO-1 proteins in ARPE-19 cells among Vehicle (0.005% DMSO), TGFβ1 (10 ng/mL) + Vehicle (0.005% DMSO), and TGFβ1 + FH535 (0.5 μmol/L) groups after treatment for 48 h. DAPI (blue). Scale bars = 100, 75 or 50 μm. B Representative images and C quantitative analysis of the number of migrating ARPE-19 cells subjected to the transwell migration assay after treatment of TGFβ1 with or without FH535 for 48 h. Scale bar = 100 µm. D Representative images depicting the scratch wound healing assay of ARPE-19 cells were captured at 0 h, 24 h, and 48 h after incubation of TGFβ1 in the presence or absence of FH535. The black lines showed the edge of the wound. E Statistical graph of scratch wound healing assay of ARPE-19 cells among above different groups. TGFβ1, transforming growth factor beta 1; ZO-1, zonula occludens-1. All results performed above are presented as mean ± SE from three independent experiments. ****p < 0.0001 compared with the TGFβ1-treated group