Table 1 The information of human primers
Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
α-SMA | CAGAAGGAGATCACGGCCCTAG | CGGCTTCATCGTATTCCTGTTTG |
collagen I | AATGTGGTTCGTGACCGTGA | AGCCTTGGTTGGGGTCAATC |
Dvl2 | TGGGGCTTCAGACCAGGATA | GCCCCCACCATAGGTGTAAG |
Dvl3 | TGCTGATAACCCATCGGAGC | TGGGCAGACACCAAAGAGTC |
fibronectin | AAGACCATACCCGCCGAATG | GGCATTTGGATTGAGTCCCG |
FZD1 | GAAAGTGCAGTGTTCCGCTG | TCACACTTGAGCGTGTCTGG |
FZD2 | CTATCCGCTGGTGAAGGTGC | CTCACAGATAGAGCGGCACGG |
FZD3 | TGATGGCTCTCATAGTTGGCA | ACCTGTCGGCTCTCATTCAC |
FZD4 | GTCTCAGTCTGGGGTTGCTC | ACGTTGTAGCCGAGGTTCTG |
GAPDH | CAAATTCCATGGCACCGTCA | GACTCCACGACGTACTCAGC |
ITGA5 | GGTCGGGGGCTTCAACTTA | AGCACACTGACCCCGTCTG |
MMP2 | TGATGGCATCGCTCAGATCC | GGCCTCGTATACCGCATCAA |
NAKED1 | AGGGGGAATAGGTGAGACCC | GGAATCCCATTGGCTGGTCA |
NAKED2 | CTTTCCGGGAGGACCAGTGT | GCACTGGAGTGCGTCAATGT |
Snail1 | TGCAGGACTCTAATCCAGAGTTT | GGACAGAGTCCCAGATGAGC |
Transgelin | AAGCGCAGGAGCATAAGAGG | ACTGATGATCTGCCGAGGTC |
Wnt1 | CAAGATCGTCAACCGAGGCT | TCACACGTGCAGGATTCGAT |
Wnt3a | ACAAAGCTACCAGGGAGTCG | TCCCACCAAACTCGATGTCC |
Wnt5a | CGGTGTACAACCTGGCTGAT | GCGCTGTCGTACTTCTCCTT |