Fig. 4
From: Inflammation-induced TRPV4 channels exacerbate blood–brain barrier dysfunction in multiple sclerosis
Enhanced TRPV4 expression in human brain ECs intensifies inflammatory phenotype during inflammatory insult. a CLDN5 and VE-Cad in TRPV4 OE compared to EV cells under homeostatic (control) and inflammatory [TNFα/IFNγ (T/I)] conditions normalized to EV control. b TEER measurement of TRPV4 OE and EV brain ECs under inflammatory conditions normalized to resistance plateau and quantification of declining slope relative to EV cells, N = 6. c Heatmap visualizes gene expression profile of TRPV4 OE and EV brain ECs under homeostasis and inflammation. Accessed categories cover transporters, immune cell migration, inflammatory and EndMT markers, N = 3 d–f Examples of differentially expressed targets were re-plotted as bar graphs to visualize effect size. Data represents mean ± SEM. Comparison of four groups was performed using one-way ANOVA with Bonferroni multiple comparisons test or non-parametric Kruskal–Wallis test with Dunn’s test (*p < 0.05; **p < 0.01; ***p < 0.001)