Table 2 Primary antibody details
From: Inflammation-induced TRPV4 channels exacerbate blood–brain barrier dysfunction in multiple sclerosis
Target | Host | Dilution | Retrieval/fixation | Supplier | Cat.# | Use |
|---|---|---|---|---|---|---|
CD3 (F7.2.38) | Mouse | 1:50 | None | Dako | M7254 | human T cells (ICC) |
CD206 | Mouse | 1:50 | Acetone | Biolegend | 321,116 | IHC FF |
CLDN5 | Rabbit | 1:200 | None | Invitrogen | #34–1600 | hCMEC/D3 |
Collagen IV | Rabbit | 1:200 | Acetone | Abcam | ab6581 | IHC FF |
GAPDH | Goat | 1:500 | None | Santa Cruz | SC-20357 | WB hCMEC/D3 |
HLA-DR (LN3) | Mouse | 1:500 | Citrate, Tris, Acetone | In house | IHC FFPE, FF | |
IBA1 | Goat | 1:500 | Citrate,Tris | Abcam | AB5076 | IHC FFPE |
P2RY12 | Rabbit | 1:100 | Citrate,Tris | Atlas antibodies | HPA014518 | IHC FFPE |
P65 | Rabbit | 1:500 | PFA (4%) | Abcam | ab16502 | ICC hCMEC/D3 |
Phalloidin | 1:100–250 | None | Molecular Probes | A12379, R415 | ICC hCMEC/D3 | |
TNFα | Rabbit | 1:200 | Acetone | Origene | PP1071P1 | IHC FF |
TRPV4 intrac | Rabbit | 1:50 | Acetone | Alomone Labs | ACC034 | IHC FF,WB |
TRPV4 extrac | Rabbit | 1:50 | Acetone | Alomone Labs | ACC124 | IHC FF |
TRPV4 | Rabbit | 1:100 | Acetone | Invitrogen | # PA5-41066 | IHC FF |
UEA-1 | 1:1000 | Acetone | Vector labs | B-1065 | IHC FF |