Fig. 5

The pre-stimuli of 1xLPS or 4xLPS change MPTP-induced motor impairments and dopaminergic damage to the nigrostriatal axis in mice. A The results of behavioral tests in mice 2 days post-MPTP administration. Time to turn around, climb down and the total time in the Pole test, and the rearing count over a 3-min period in the Rearing test were shown. n = 6–10/group. The difference shown by “++” was analyzed by unpaired t test. ⁺⁺p < 0.01. B The protein levels of TH and GFAP in mouse striatum detected by Western Blot. n = 4–8 /group. C Assessments of the optical density of TH⁺ nerve fibers in the striatum of mice by immunohistochemistry staining. Scale bar, 200 μm. n = 4/group. D Assessments of TH⁺ neurons in the SNpc of mice by immunohistochemistry staining and stereological counting. Scale bar, 100 μm. n = 4/group. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001. The difference shown by “+” was analyzed by unpaired t test. ⁺p < 0.05. ns, no significant difference