Fig. 6

The expression of HIF-1α in striatal microglia of chronic innate immune memory model mice treated with NS or MPTP. A Representative images of IBA1/HIF-1α/CD16/32 triple immunostaining in the striatum of MPTP-induced PD mice with innate immune memory. Scale bar, 50 µm. Small plots in the upper left quadrant showed the zoomed details. Scale bar, 20 µm. The experimental schedule was described as in Fig. 3A, B. Quantification of the number of HIF-1α⁺, CD16/32⁺ and HIF-1α⁺ CD16/32⁺ cells in all IBA1-immunolabeled cells from mouse striatum, 7 days post-MPTP administration. n = 4/group. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001. C The relative proportion of IBA1-immunolabeled cells in MPTP-induced PD mice based on innate immune memory models. The changes in the proportion of HIF-1α⁻ CD16/32⁻ microglia in the experimental groups compared to Control groups was marked by asterisks (*) within the figure, and differences between these groups was indicated by pound sign (#). n = 4/group. Differences were analyzed by one-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001 vs control groups. ^###p < 0.001. D Pearson’s correlation analysis showing density of HIF-1α⁺ and CD16/32⁺ cells in all of the IBA1-immunolabeled microglia. Pearson's correlation coefficient (r) and p-values are shown above