Fig. 7

Acute innate immune memory models cannot be established in microglial Hif-1α conditional knockout mice. A Experimental strategy for tamoxifen-induced deletion of Hif-1α specifically in microglia. B The changes of body weight during LPS injections. n = 5–8/group. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, ***p < 0.001. The experimental schedule was the same as Fig. 2A. (Ctrl: Cre-negative control littermates; cKO: Hif-1α cKO). C Transcription of pro-inflammatory cytokines (Il-1β, Il-6 and Tnf-α) and anti-inflammatory cytokines (Il-4 and Il-10) in the striatum of Ctrl and cKO mice detected by RT-qPCR. n = 3–5 /group. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. **p < 0.01, ***p < 0.001 vs normal saline (NS) control. ^##p < 0.05, ^###p < 0.001 vs Ctrl mice. ^^p < 0.01, ^^^p < 0.001. D Representative images of IBA1 (red) immunostaining in the striatum of mice 3 h after NS, 2xLPS or 4xLPS injection. Scale bar, 100 µm. Quantity analysis of IBA1⁺ cells in the dorsal striatum is shown in the right panel. n = 3–4/group. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, ***p < 0.001 vs normal saline (NS) control. #p < 0.05, ##p < 0.01 vs Cre-negative control. ^^p < 0.01