Fig. 9

Hif-1α deficiency in microglia ameliorates MPTP-induced damage in the nigrostriatal pathway in mice. A–C The results of behavioral tests in mice 2 days post-MPTP administration. Time to turn around, climb down and the total time in the Pole test (A), the rearing counts in a 3-min period in the Rearing test (B), and the latency time to fall and scores in the Wire hanging test (C) were showed. n = 7–13 /group. D Protein levels of TH in mouse striatum detected by Western Blot. n = 5–6/group. The differences indicated by “+” were analyzed by unpaired t test. E Assessments of the optical density of TH⁺ nerve fibers in the striatum of mice by Immunohistochemistry staining. Scale bar, 200 μm. n = 3–4/group. F Assessment of TH⁺ neurons in the SNpc of mice by immunohistochemistry staining and stereological counting. Scale bar, 50 μm. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001 vs normal saline (NS) control. ^##p < 0.01 vs Cre-negative control. The difference shown by “+” was analyzed by unpaired t test. ⁺p < 0.05