Fig. 2From: Single-cell RNA sequencing reveals the immune features and viral tropism in the central nervous system of mice infected with Japanese encephalitis virusFlow cytometry analysis of immune cells in brains of mice with different symptoms upon JEV infection. A, C, E, G Flow cytometry gating strategy of Mic_Mac (A), Mon_Mac (C), NK cells (E) and T cells (G). B Percentage of CD11b + CD45low cells in total living cells and percentage of Cx3cr1 + cells in Mic_Mac cells. D Percentage of CD11b + CD45high cells in total living cells and the number of cells that secrete MHC-II. F Percentage of CD49+ CD3-CD45+ cells in total living cells. H Cell numbers of CD3+, CD4+ CD3+, and CD8a + CD3+ cells. Data from 3 independent experiments were presented as mean ± SEM. The statistics were analyzed using two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns: nonsignificantBack to article page