Fig. 6

Baiap2 positive neurons exhibit high susceptibility to JEV. A Distribution of JEV genome in different cells. B Percentage of JEV-infected cells (the count of JEV gene > 500) in each cell type. C Flow cytometry for detection of JEV E protein and NeuN in brain cells of mice infected with JEV. D The histograms summarizing the indicated cell populations of JEV-infected neurons (n = 4). E tSNE visualization of clustering revealed 15 distinct neuronal populations. F Histogram showing the proportions of cells in each group and each subcluster. G Dot plot showing the expression of representative markers that define the cluster in neurons. H tSNE visualization of JEV gene expression in neuron cells and heatmap of JEV transcript positive cells separated by different symptoms. I Heatmap showing the neurons with different abundance of JEV genomic RNA. J The top 5 genes in brain cells infected with different amount of JEV. Different color dots represent viral gene expression, yellow dot: low expression (Log2 Exp JEV less than 3); green dot: medium expression (Log2 Exp JEV between 3 and 8); red dot: high expression (Log2 Exp JEV more than 8). K Heatmap of Baiap2 expression in neurons in mouse brain. L Flow cytometry analysis of JEV and Baiap2 positive cells in brains of mice with different symptoms after JEV infection. M The histograms summarizing the ratios of indicated cells infected with JEV in the Baiap2 positive neurons (n = 4). N The histograms summarizing the ratio of Baiap2 and JEV positive cells in NeuN and JEV positive cells. Data from 3 independent experiments were presented as mean ± SEM. The statistics were analyzed using two-tailed Student’s t-test. ns: nonsignificant