Fig. 4

Microglia ramification is unaffected by cranial irradiation. A Example image of an individual microglia. Scale bar = 20 microns B Image of the same microglia after thresholding C Representation of Sholl analysis demonstrating concentric circles drawn at increasing radii from the center of the soma D Sholl curves for control and irradiated mice at baseline and Week 4. Sholl curves for E control and F irradiated mice at different time points. G Percent change in maximum number of intersections for control and irradiated mice over time. H Percent change in mean number of intersections for control and irradiated mice over time. I Percent change in area under the curve for control and irradiated mice over time. Mixed-effects analysis with Bonferroni’s post-hoc comparisons (G–I). Total cells analyzed per group: Control = 158, IR = 126. Data are presented as mean ± SEM. Each data point represents an individual animal. Sholl curves in D–F are presented as the mean for each group and timepoint specified. n = 8–11 mice per timepoint per group