Fig. 5

Microglia morphology is unaffected by cranial irradiation. A Example image of an individual microglia. Scale bar = 20 microns B Image of the same microglia after thresholding representing the whole microglial cell. C The microglial soma and processes were identified manually. D Percent change in microglia size for control and Irradiated mice over time. E Percent change in process size for control and irradiated mice over time F Percent change in soma size for control and irradiated mice over time G Percent change in soma to process ratio for control and irradiated mice over time Mixed-effects analysis with Bonferroni’s post-hoc comparisons (D–G). Total cells analyzed per group: Control = 158, IR = 126. Data are presented as mean ± SEM. Each data point represents an individual animal. n = 8–11 mice per timepoint per group.