Fig. 6

Microglia dynamics are altered following cranial irradiation. A Representative images used for dynamic measurements of microglia in the same area of cortex at different time points. Scale bar = 25 microns. B Representative image of binarized microglia at time = 0 min (T0) in magenta, time = 5 min (T5) in green, and merged timepoints. White represents pixels that are stable for both timepoints, while magenta represents retracted processes and green represents extended processes. C Percent change in microglial coverage for control and Irradiated mice over time. D Percent change in microglial surveillance index for control and irradiated mice over time. E Percent change in microglial motility Index for control and irradiated mice over time. F Percent change in microglial motility to coverage ratio for control and irradiated mice over time. G) Percent change in microglial motility to surveillance ratio for control and irradiated mice over time. Mixed-effects analysis with Bonferroni’s post-hoc comparisons (C–G). * = p < 0.05, ** = p < 0.01. Data are presented as mean ± SEM. Each data point represents an individual animal. n = 9–11 mice per timepoint per group