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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Pathological high intraocular pressure induces glial cell reactive proliferation contributing to neuroinflammation of the blood-retinal barrier via the NOX2/ET-1 axis-controlled ERK1/2 pathway

Fig. 2

Pathologically high intraocular pressure-induced glaucomatous RGC loss and neurodegeneration. (A) Representative images of flat mount retina immunostained with Brn3a after Pressure 60 mmHg. Scale bar, 50 μm. (B) Analysis of the number of Brn3a-positive RGCs at different times after Pressure 60 mmHg. Data are shown as mean ± SEM (n = 6 in each group, one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05,**p < 0.01, ***p < 0.001, ****p < 0.0001). (C) Time course of IOP before as well as 14 days after H-IOP surgery. Data are shown as mean ± SEM (n = 6 in each group, two-way ANOVA with Šídák’s multiple comparisons test, ****p < 0.0001). (D) Representative images of flat mount retina immunostained with Brn3a and PPD-stained ON after H-IOP. Scale bar, 50 μm and 5 μm. (E) Analysis of the number of Brn3a-positive RGCs at different times after H-IOP. (F) Analysis of the number of axons and the percent of degenerating axons in the different groups. Data are shown as mean ± SEM (n = 6 in each group, one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05,**p < 0.01, ***p < 0.001, ****p < 0.0001). (G) Messenger RNA expression of Nox enzymes (Nox2, p47phox, Nox1, Nox4) in H-IOP retinas. Data are presented as the fold-change after H-IOP versus control. Data are shown as mean ± SEM (n = 6 in each group, Unpaired T-test, *p < 0.05,**p < 0.01, ***p < 0.001, ****p < 0.0001). (H) Representative images of retinal cross-sections immunostained with NOX2 after Pressure 60 mmHg (Scale bar, 50 μm) and the analysis of NOX2 fluorescence intensity in the retinal vessels and different retinal layers. (I) Representative images of retinal cross-sections immunostained with DHE staining after Pressure 60 mmHg. Scale bar, 50 μm. Analysis of DHE fluorescence intensity in the retinal vessels and different retinal layers. The white arrows point to cross-sections of retinal blood vessels. Data are presented as the percent fluorescence intensity of the Pressure 60 mmHg versus control. Data are shown as mean ± SEM (n = 6 in each group, Unpaired T-test, **p < 0.01, ****p < 0.0001)

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Journal of Neuroinflammation

ISSN: 1742-2094