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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Pathological high intraocular pressure induces glial cell reactive proliferation contributing to neuroinflammation of the blood-retinal barrier via the NOX2/ET-1 axis-controlled ERK1/2 pathway

Fig. 6

NOX2/ROS-activated glial cells involved in H-IOP-mediated blood-retinal barrier damage and inflammation. (A) Representative images of the iBRB stained by GS-IB4, α-SMA, and GFAP. Scale bar, 50 μm. (B) Analysis of the α-SMA positive % area and the α-SMA positive % area/GS-IB4 positive % area in the different groups (n = 6 in each group). (C) GFAP positive percentage area in the different groups (n = 6 in each group). (D) Representative immunostaining of Iba1 and GS-IB4 images of retinas after H-IOP treated by gp91ds-tat. Scale bar, 50 μm. (E) Analysis of Iba1 positive percentage area in the different groups (n = 6 in each group). (F) Representative immunostaining of CD31 images of retinas after H-IOP treated by gp91ds-tat. Scale bar, 100 μm. (G) Analysis of the vascular area fraction, the number of branches/mm2, and the main vessel diameter (µm) in the different groups (n = 6 in each group). Data are shown as mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05,**p < 0.01,***p < 0.001, ****p < 0.0001

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Journal of Neuroinflammation

ISSN: 1742-2094