Table 1 Antibodies used for histological analyses
Marker | Company | Dilution | Secondary antibody |
|---|---|---|---|
Brn3a (mouse) | Millipore, MAB1585, Lot#3,684,607, EMD Millipore Corporation, Temecula, CA, USA | 1:200 | goat anti-mouse IgG H + L (Alexa Flour 488; ab150113, Abcam, Cambridge, UK) |
Iba1 (rabbit) | 019-19741, FUJIFILM Wako Pure Chemical Corporation | 1:500 | goat anti-rabbit IgG H + L (Alexa Flour 594; ab150080, Abcam, Cambridge, UK) |
CD31 (mouse) | MEC13.3, BD | 1:200 | goat anti-mouse IgG H + L (Alexa Flour 488; ab150113, Abcam, Cambridge, UK) |
NOX2 (rabbit) | ab129068, Abcam, Cambridge, UK | 1:200 | goat anti-rabbit IgG H + L (Alexa Flour 594; ab150080, Abcam, Cambridge, UK) |
ET-1 (mouse) | NB300-526, Novus Biologicals, Littleton, USA | 1:200 | goat anti-mouse IgG H + L (Alexa Flour 488; ab150113, Abcam, Cambridge, UK) |
GAFP (rabbit) | #Z0334, Agilent (DAKO) | 1:200 | donkey anti-rabbit IgG H + L (Alexa Flour 647; ab150075, Abcam, Cambridge, UK) |
Isolectin GS-IB4 | Alexa Fluor 488 conjugate, Invitrogen | 1:500 | - |
α-SMA | ab202368 Abcam, Cambridge, UK Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody (1A4) | 1:200 | - |
p-ERK1/2 (rabbit) | #9101, Cell Signaling | 1:300 | goat anti-rabbit IgG H + L (Alexa Flour 594; ab150080, Abcam, Cambridge, UK) |