Table 2 Primer sequences used for quantitative PCR analysis
Gene | Accession number | Forward | Reverse |
|---|---|---|---|
Nox1 | NM_172203.1 | GGAGGAATTAGGCAAAATGGATT | GCTGCATGACCAGCAATGTT |
Nox2 | NM_007807.2 | CCAACTGGGATAACGAGTTCA | GAGAGTTTCAGCCAAGGCTTC |
p47 phox | NM_001286037.1 | AGAGCACGGATGGCACAAAG | CCGCGGGCTGTGGTT |
Nox4 | NM_015760.2 | GGCTGGCCAACGAAGGGGTTAA | GAGGCTGCAGTTGAGGTTCAGGACA |
Ho-1 | NM_010442 | GGTGATGCTGACAGAGGAACAC | TAGCAGGCCTCTGACGAAGTG |
Vegf-a | NM_001025250.3 | ACTTGTGTTGGGAGGAGGATGTC | AATGGGTTTGTCGTGTTTCTGG |
Gpx1 | NM_008160 | CCCGTGCGCAGGTACAG | GGGACAGCAGGGTTTCTATGTC |
Sod2 | NM_013671 | CCTGCTCTAATCAGGACCCATT | CGTGCTCCCACACGTCAAT |
Tnf-α | NM_001278601.1 | GCCTCTTCTCATTCCTGCTTG | CTGATGAGAGGGAGGCCATT |
Il-1β | NM_008361 | AAGGAGAACCAAGCAACGACAAAA | TGGGGAACTCTGCAGACTCAAACT |
eNos | NM_008713 | CCTTCCGCTACCAGCCAGA | CAGAGATCTTCACTGCATTGGCTA |
iNos | NM_010927 | CAGCTGGGCTGTACAAACCTT | CATTGGAAGTGAAGCGTTTCG |
nNos | NM_008712 | TCCACCTGCCTCGAAACC | TTGTCGCTGTTGCCAAAAAC |
Tbp | NM_013684 | CTTCGTGCAAGAAATGCTGAAT | CAGTTGTCCGTGGCTCTCTTATT |