Fig. 2
From: Impact of microglia isolation and culture methodology on transcriptional profile and function
Analysis of microglial isolation metrics. A–C Bar graphs highlight microglial yield, RNA yield and RNA integrity across Protocols 1A, 1B, 1C, 2, and 3. A Total microglia yield as measured by hemocytometer, per 5 pups or 1 adult. B RNA yields from Nanodrop, displayed as ngRNA/100,000 lysed cells. C RNA integrity score assessed by Bioanalyzer. Asterisks indicate p values of most relevant comparisons, determined by a two-tailed t-test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001