Fig. 2

CD27 cleavage is triggered by TCR activation. (A) Western blot analysis of CD27 in whole cell lysate from CD4 and CD8 T cells in resting and stimulated with 5μg/ml of anti-CD3 Ab at 72 h. Quantification of CD27 as a percentage of control and sCD27 as a value relative to normalization with GADPH in CD4 (B) and CD8 (C) T cells by western blotting. Data were reported as mean ± SD of 3 independent experiments. Statistical analyses were performed using One sample t and Wilcoxon test. NS = not significant, *p < 0.05, **p < 0.01. Levels of sCD27 and IFNγ- released from CD4 (D, F) and CD8 (E, G) T cells activated with concentrations of anti-CD3 including 1, 5 and 10 ug/ml at 72h and 96h as measured by ELISA. Statistical analyses were performed using Tukey’s multiple comparison tests two-way ANOVA (D, F) and Tukey’s multiple comparison test for mixed-effects analysis (E, G). Data represent mean ± SD of 6 (D, F) and 4 independent experiments (E, G).*p < 0.05,**p < 0.01,****p < 0.0001.