Fig. 1

Sex-dependent expression of P2RY12 in adult microglia. A Shows a three-dimensional reconstructed image of microglia demonstrating the specificity of P2RY12 antibody labeling in CX3CR1^−GFP/+ reporter mice scale bar: 3 µm. B Provides sample cortical immunofluorescent images confirming the absence of P2RY12 reactivity in adult CX3CR1^−GFP/+: P2RY12^−/− mice scale bar: 20 µm. (C) Presents flow cytometric analysis verifying the absence of P2RY12 from development through to adulthood in CX3CR1^−GFP/+: P2RY12^−/− mice. D Details P2RY12 expression in microglia by sex, and (E) document P2RY12 expression in female mice across the estrous cycle. Data in panel C and D were analysed using student’s t-test, and data in E were analysed using one-way ANOVA with Tukey’s post hoc test. Data presented are reported as mean ± SEM. A p-value < 0.05 denotes statistical significance. N = 3–5 for C N = 4–9 per group for D, and N = 3–7 per group for panel E. **p signifies p < 0.01; ***p signifies p < 0.001, ****p signifies p < 0.0001, with the symbols indicating levels of statistical significance