Fig. 2

P2RY12 deficiency results in an increase in microglia population density. A, B Display representative maximum intensity projections showcasing microglia density in the dorsal striatum of adult female and male mice. Scale bar: 20μm.  C–E Indicate an increase in microglia density in the cortex, hippocampal CA1, and dorsal striatum for both female and male mice lacking P2RY12. F–H Demonstrate that the average distance to the three nearest neighbors is reduced in the specified brain region in both female and male P2RY12-deficient mice. Data analysis was performed with 2-way ANOVA to assess the effects of both genotype and sex and presented as mean ± SEM. Tukey’s multiple comparisons test was applied for post hoc analysis. A p-value < 0.05 was considered significant, with levels of significance indicated by: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. N = 3–4 mice per group