Fig. 7

Long-term reconstitution of bone marrow cells transplanted from chronic TBI mice worsens ongoing neurodegeneration at the transcriptomic level in host brains. The transcriptomic profile of whole brain hemisphere tissue at 8 months after irradiation was assessed using the NanoString nCounter Neuropathology panel. (A) Chimera paradigm is illustrated. (B) Partial least squares-discriminant analysis (PLSDA) plot showed a separation of clusters between the TBI→WT (T) and SH→WT (S) groups. (C) Pathway enrichment analysis with the MSigDB Hallmark 2020 database revealed that several gene networks related to apoptosis, oxidative phosphorylation, UV response, and complement pathways were enriched in TBI→WT compared to SH→WT mice. (D) Volcano plot analyses are shown between two groups. (E) Heat map analysis of the top 20 most differentially expressed genes are indicated. Color coding was based on z-score scaling. (F) Normalized transcription count of the top 9 genes presented as Log10 abundance. n = 4 mice/group