Fig. 8

The acute neuroinflammatory response to a subsequent TBI is sensitized with increasing time post-injury. (A) Chimera paradigm is illustrated. Mice reconstituted for 8 weeks following transplantation were subjected to TBI. (B) Representative dot plots of immune populations in the ipsilateral brain hemisphere at 48 h after TBI. (C-D) Quantification of CD45^intCD11b⁺ microglia and CD45^hiCD11b⁺-infiltrating myeloid cell counts per hemisphere are shown for Sham and TBI in chimeric mice. n = 4–8 mice/group. (E) Chimeric mice reconstituted for 8 months following transplantation were subjected to TBI. (F) A representative dot plot of leukocyte populations in the ipsilateral brain at 48 h after TBI. (G-H) Quantification of CD45^intCD11b⁺ microglia (G) and CD45^hiCD11b⁺ myeloid cells (H) are shown. n = 6–8 mice/group. Data were analyzed using two-way ANOVA group analysis with Tukey’s test for multiple comparisons. **p < 0.01, *p < 0.05. SH: Sham