Constitutive knockout of interleukin-6 ameliorates memory deficits and entorhinal astrocytosis in the MRL/lpr mouse model of neuropsychiatric lupus

Table 2 Histologic glial cell quantification in the brain

Condition	Genotype	GFAP + cells in s.sensory cx	GFAP + cells in amygdala	GFAP + cells in dentate gyrus	GFAP + cells in entorhinal cx
IL-6 WT	IL-6^+/+ MRL/lpr	15 ± 7	57 ± 18	125 ± 25	148 ± 25
IL-6 KO	IL-6^−/− MRL/lpr	10 ± 2	37 ± 9	140 ± 45	89 ± 16
p-value	–	0.476	0.172	0.211	0.037*

Condition	Iba1 + cells in retrosplenial cx	Iba1 + cells in s.sensory cx	Iba1 + cells in amygdala	Iba1 + cells in dentate gyrus	Iba1 + cells in entorhinal cx
IL-6 WT	28 ± 2	21 ± 1	28 ± 2	10 ± 1	53 ± 3
IL-6 KO	37 ± 6	24 ± 1	28 ± 1	12 ± 1	47 ± 4
p-value	0.092	0.500	0.396	0.070	0.106

Quantification of the density (cells/mm²) of GFAP + and Iba1 + cells in key brain regions. Cohort A was used for gene expression analyses, Cohort B was used for histologic analysis. Mean density ± standard error for each outcome measured. Within-genotype outcomes were statistically equivalent
cx, cortex; s.sensory, somatosensory
Bold p-values are significant. *p < 0.05

ISSN: 1742-2094