Fig. 1

After stroke, STING was primarily expressed and upregulated in microglia. A Representative bands of STING and β-ACTIN using Western Blotting experiments. B The quantification of STING expression levels with β-ACTIN as the internal reference. The expression levels were relative to sham-treated group. n = 4 mice per condition. C The protein levels of IFNβ detected by ELISA. n = 5–6 mice. Each dot represented an individual mouse. D Representative immunofluorescent micrographs of STING and IBA1 at different time points after photothrombotic stroke. E The quantification of STING-positive microglia. Microglia were labeled by IBA1. F The mean fluorescence intensity (MFI) of STING relative to sham-treated group. G The quantification of STING-positive area. n = 7–9 field of view (FOV) from 3 mice per condition. Each dot represented a FOV. H and I Representative immunofluorescent micrographs of STING and neuronal marker NeuN (H) and astrocytic marker GFAP (I). Boxed regions were enlarged for analyzing colocalization. Scale bar = 20 μm (D and I). Scale bar = 10 μm (H). Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001