Fig. 8

H151 inhibited STAT1 expression and nucleus translocation. A Chordal graph showing the regulatory relationship between transcriptional factors and phagocytosis-related molecules based on the database of Cistrome Data Browser. B Heat map of the mRNA levels of the transcriptional factors that potentially regulated phagocytosis. Darker color represented higher fold change. ^##P < 0.01, ^###P < 0.001, compared with sham + vehicle group; *P < 0.05, compared with PT + vehicle group. n = 4–6 mice per condition. C Representative bands of STAT1 and SPI1 in each experimental condition. D, E Quantitive analysis of protein expression levels STAT1 (D) and SPI1 (E). n = 6 mice per condition. F Representative immunofluorescent micrographs and 3D reconstructions of phosphorylated STAT1, IBA1, and Hoechst. Scale bar = 5 μm. G The relative p-STAT1 puncta number in the nucleus of IBA1-positive cell. n = 19–74 cells from 3 mice. Each dot represented an analyzed cell. H Representative bands of phospho-STAT1 and Lamin B1. The nuclear fractions were used for Western Blotting. I Quantitive analysis of protein expression levels of phospho-STAT1. Lamin B1 was used as internal control for nuclear protein. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant