Fig. 2

Double immunohistochemical analysis of NKT cells in the brains of ⍺-synuclein transgenic mice. (A) Split and merged representative microscopy images from the neocortex of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d double labeled with antibodies against CD3 (red channel) and IFN𝛾 (FITC channel), co-localizing CD3 and IFN𝛾 (merged images, yellow) suggests representing of NKT cells. (B) Split and merged representative microscopy images from the neocortex of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d double labeled with antibodies against CD3 (red channel) and CD1d (FITC channel), showing the relative proximity of T cells to the processes of glial cells expressing CD1d. (C) Split and merged representative microscopy images from the neocortex of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d double labeled with antibodies against CD1d (red channel) and GFAP (FITC channel). (D) Split and merged representative microscopy images from the neocortex of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d double labeled with antibodies against CD3 (red channel) and GFAP (FITC channel), showing the relative proximity of T cells to the processes of glial cells expressing GFAP. Scale bars = 15 μm (low magnification) and 5 μm (high magnification)