Fig. 4

Immunohistochemical analysis of astrocytes and microglial cells in the brains of ⍺-synuclein transgenic mice treated with anti-CD1d. (A) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d immunostained with an antibody against GFAP (astrocytes marker). (B–D) Computer based image analysis showing increased GFAP immunoreactivity in the neocortex, hippocampus, and striatum of ⍺-syn tg mice and decreased levels with anti-CD1d treatment. (E) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG and anti-CD1d immunostained with an antibody against Iba-1 (microglial cell marker). (F–H) Computer based image analysis showing increased numbers of Iba-1 positive cells in the neocortex, hippocampus, and striatum in IgG and anti-CD1d treated non-tg and ⍺-syn tg mice. Scale bar = 50 μm (high magnification). n = 4 mice per group. Statistical significance determined by two-way ANOVA with post-hoc Tukey; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001