Fig. 5

Immunohistochemical analysis of microglial activation in the brains of ⍺-synuclein transgenic mice treated with anti-CD1d. (A) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d immunostained with an antibody against TMEM119 (microglia cell marker). (B–D) Computer based image analysis showing the levels of TMEM119 immunoreactivity in the neocortex, hippocampus and striatum in IgG and anti-CD1d treated non-tg and ⍺-syn tg mice. (E) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d immunostained with an antibody against CCL4 (cell marker). (F–H) Computer based image analysis showing the levels of CCL4 immunoreactivity in the neocortex, hippocampus, and striatum in IgG and anti-CD1d treated non-tg and ⍺-syn tg mice. Scale bar = 10 μm (high magnification). n = 4 mice per group. Statistical significance determined by two-way ANOVA with post-hoc Tukey; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001